anti flag m2 affinity resin Search Results


93
Bio-Rad star145 goat anti human igm polyclonal antibody
Star145 Goat Anti Human Igm Polyclonal Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
Abcam anti inflammatory m2 macrophages cd86
Anti Inflammatory M2 Macrophages Cd86, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson taz (m2-616
Taz (M2 616, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson rabbit anti-arginase 1
(A) Representative double-immunofluorescence of CD16/32 (classical activation marker; red), CD206 or <t>arginase</t> <t>1</t> (ARG1, alternative activation markers; red) and Iba1 (green) in the contusion margin (see the red box in the brain atlas coronal brain section in Figure ) of WT and sEH KO mice at 1 day and 4 days following TBI. Arrowheads indicate the cells of colocalization. The inset images represent higher magnification of the boxed region in the corresponding images. The scale bar is 100 μm. The bar graph shows the degree of Iba1 and CD16/32, CD206 or arginase 1 colocalization in gray pixel intensity. Deletion of sEH resulted in a significant decrease of CD16/32-Iba1double-positive signal at 1 day and 4 days and an increase of CD206-Iba1 or arginase-1 double-positive signal at 4 days post-TBI. (B) Bar graphs of IL-4 protein level as assessed by ELISA in ipsilateral hemispheres of WT and sEH KO mice at 1 day and 4 days following sham surgery or TBI. Deletion of sEH caused a significant elevation of IL-4 level at 4 day. Values are mean ± S.E.M; ### , ††† P < 0.001 vs. sham group; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. WT group (n = 5 mice / group for CD16/32, CD 206 or arginase 1 and Iba1 double stainings, Student’s t -test; n = 6 mice / group for IL-4 ELISA, repeated measures two-way ANOVA).
Rabbit Anti Arginase 1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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rrm2  (Abnova)
90
Abnova rrm2
(A) Representative double-immunofluorescence of CD16/32 (classical activation marker; red), CD206 or <t>arginase</t> <t>1</t> (ARG1, alternative activation markers; red) and Iba1 (green) in the contusion margin (see the red box in the brain atlas coronal brain section in Figure ) of WT and sEH KO mice at 1 day and 4 days following TBI. Arrowheads indicate the cells of colocalization. The inset images represent higher magnification of the boxed region in the corresponding images. The scale bar is 100 μm. The bar graph shows the degree of Iba1 and CD16/32, CD206 or arginase 1 colocalization in gray pixel intensity. Deletion of sEH resulted in a significant decrease of CD16/32-Iba1double-positive signal at 1 day and 4 days and an increase of CD206-Iba1 or arginase-1 double-positive signal at 4 days post-TBI. (B) Bar graphs of IL-4 protein level as assessed by ELISA in ipsilateral hemispheres of WT and sEH KO mice at 1 day and 4 days following sham surgery or TBI. Deletion of sEH caused a significant elevation of IL-4 level at 4 day. Values are mean ± S.E.M; ### , ††† P < 0.001 vs. sham group; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. WT group (n = 5 mice / group for CD16/32, CD 206 or arginase 1 and Iba1 double stainings, Student’s t -test; n = 6 mice / group for IL-4 ELISA, repeated measures two-way ANOVA).
Rrm2, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+flag+m2+affinity+resin/rrm2+antibody/pmc06076438-163-35-36
Average 90 stars, based on 1 article reviews
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96
Novus Biologicals rat anti flag
(A) Representative double-immunofluorescence of CD16/32 (classical activation marker; red), CD206 or <t>arginase</t> <t>1</t> (ARG1, alternative activation markers; red) and Iba1 (green) in the contusion margin (see the red box in the brain atlas coronal brain section in Figure ) of WT and sEH KO mice at 1 day and 4 days following TBI. Arrowheads indicate the cells of colocalization. The inset images represent higher magnification of the boxed region in the corresponding images. The scale bar is 100 μm. The bar graph shows the degree of Iba1 and CD16/32, CD206 or arginase 1 colocalization in gray pixel intensity. Deletion of sEH resulted in a significant decrease of CD16/32-Iba1double-positive signal at 1 day and 4 days and an increase of CD206-Iba1 or arginase-1 double-positive signal at 4 days post-TBI. (B) Bar graphs of IL-4 protein level as assessed by ELISA in ipsilateral hemispheres of WT and sEH KO mice at 1 day and 4 days following sham surgery or TBI. Deletion of sEH caused a significant elevation of IL-4 level at 4 day. Values are mean ± S.E.M; ### , ††† P < 0.001 vs. sham group; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. WT group (n = 5 mice / group for CD16/32, CD 206 or arginase 1 and Iba1 double stainings, Student’s t -test; n = 6 mice / group for IL-4 ELISA, repeated measures two-way ANOVA).
Rat Anti Flag, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+flag+m2+affinity+resin/DYKDDDDK+Epitope+Tag+Antibody+(L5)/bio_rxiv__2021__10__20__465173-302-56-59
Average 96 stars, based on 1 article reviews
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90
Cosmo Bio USA anti-6-4pp
(A) Representative double-immunofluorescence of CD16/32 (classical activation marker; red), CD206 or <t>arginase</t> <t>1</t> (ARG1, alternative activation markers; red) and Iba1 (green) in the contusion margin (see the red box in the brain atlas coronal brain section in Figure ) of WT and sEH KO mice at 1 day and 4 days following TBI. Arrowheads indicate the cells of colocalization. The inset images represent higher magnification of the boxed region in the corresponding images. The scale bar is 100 μm. The bar graph shows the degree of Iba1 and CD16/32, CD206 or arginase 1 colocalization in gray pixel intensity. Deletion of sEH resulted in a significant decrease of CD16/32-Iba1double-positive signal at 1 day and 4 days and an increase of CD206-Iba1 or arginase-1 double-positive signal at 4 days post-TBI. (B) Bar graphs of IL-4 protein level as assessed by ELISA in ipsilateral hemispheres of WT and sEH KO mice at 1 day and 4 days following sham surgery or TBI. Deletion of sEH caused a significant elevation of IL-4 level at 4 day. Values are mean ± S.E.M; ### , ††† P < 0.001 vs. sham group; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. WT group (n = 5 mice / group for CD16/32, CD 206 or arginase 1 and Iba1 double stainings, Student’s t -test; n = 6 mice / group for IL-4 ELISA, repeated measures two-way ANOVA).
Anti 6 4pp, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+flag+m2+affinity+resin/anti++6+4+pp+antibody/pmc08290050-213-8-12
Average 90 stars, based on 1 article reviews
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95
Jackson Immuno alexa fluor 647 conjugated mouse anti m2 mab o19
(A) Representative double-immunofluorescence of CD16/32 (classical activation marker; red), CD206 or <t>arginase</t> <t>1</t> (ARG1, alternative activation markers; red) and Iba1 (green) in the contusion margin (see the red box in the brain atlas coronal brain section in Figure ) of WT and sEH KO mice at 1 day and 4 days following TBI. Arrowheads indicate the cells of colocalization. The inset images represent higher magnification of the boxed region in the corresponding images. The scale bar is 100 μm. The bar graph shows the degree of Iba1 and CD16/32, CD206 or arginase 1 colocalization in gray pixel intensity. Deletion of sEH resulted in a significant decrease of CD16/32-Iba1double-positive signal at 1 day and 4 days and an increase of CD206-Iba1 or arginase-1 double-positive signal at 4 days post-TBI. (B) Bar graphs of IL-4 protein level as assessed by ELISA in ipsilateral hemispheres of WT and sEH KO mice at 1 day and 4 days following sham surgery or TBI. Deletion of sEH caused a significant elevation of IL-4 level at 4 day. Values are mean ± S.E.M; ### , ††† P < 0.001 vs. sham group; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. WT group (n = 5 mice / group for CD16/32, CD 206 or arginase 1 and Iba1 double stainings, Student’s t -test; n = 6 mice / group for IL-4 ELISA, repeated measures two-way ANOVA).
Alexa Fluor 647 Conjugated Mouse Anti M2 Mab O19, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+flag+m2+affinity+resin/Alexa+Fluor+647+IgG+Fraction+Monoclonal+Mouse+Anti-Fluorescein/bio_rxiv__858373-178-17-34
Average 95 stars, based on 1 article reviews
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92
Bio-Rad γ globulin
(A) Representative double-immunofluorescence of CD16/32 (classical activation marker; red), CD206 or <t>arginase</t> <t>1</t> (ARG1, alternative activation markers; red) and Iba1 (green) in the contusion margin (see the red box in the brain atlas coronal brain section in Figure ) of WT and sEH KO mice at 1 day and 4 days following TBI. Arrowheads indicate the cells of colocalization. The inset images represent higher magnification of the boxed region in the corresponding images. The scale bar is 100 μm. The bar graph shows the degree of Iba1 and CD16/32, CD206 or arginase 1 colocalization in gray pixel intensity. Deletion of sEH resulted in a significant decrease of CD16/32-Iba1double-positive signal at 1 day and 4 days and an increase of CD206-Iba1 or arginase-1 double-positive signal at 4 days post-TBI. (B) Bar graphs of IL-4 protein level as assessed by ELISA in ipsilateral hemispheres of WT and sEH KO mice at 1 day and 4 days following sham surgery or TBI. Deletion of sEH caused a significant elevation of IL-4 level at 4 day. Values are mean ± S.E.M; ### , ††† P < 0.001 vs. sham group; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. WT group (n = 5 mice / group for CD16/32, CD 206 or arginase 1 and Iba1 double stainings, Student’s t -test; n = 6 mice / group for IL-4 ELISA, repeated measures two-way ANOVA).
γ Globulin, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+flag+m2+affinity+resin/Liquichek+Anti-nDNA+Control/pmc03725601-255-23-12
Average 92 stars, based on 1 article reviews
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90
Phase Holographic Imaging PHI AB digital holographic microscope holomonitortm m2
(A) Representative double-immunofluorescence of CD16/32 (classical activation marker; red), CD206 or <t>arginase</t> <t>1</t> (ARG1, alternative activation markers; red) and Iba1 (green) in the contusion margin (see the red box in the brain atlas coronal brain section in Figure ) of WT and sEH KO mice at 1 day and 4 days following TBI. Arrowheads indicate the cells of colocalization. The inset images represent higher magnification of the boxed region in the corresponding images. The scale bar is 100 μm. The bar graph shows the degree of Iba1 and CD16/32, CD206 or arginase 1 colocalization in gray pixel intensity. Deletion of sEH resulted in a significant decrease of CD16/32-Iba1double-positive signal at 1 day and 4 days and an increase of CD206-Iba1 or arginase-1 double-positive signal at 4 days post-TBI. (B) Bar graphs of IL-4 protein level as assessed by ELISA in ipsilateral hemispheres of WT and sEH KO mice at 1 day and 4 days following sham surgery or TBI. Deletion of sEH caused a significant elevation of IL-4 level at 4 day. Values are mean ± S.E.M; ### , ††† P < 0.001 vs. sham group; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. WT group (n = 5 mice / group for CD16/32, CD 206 or arginase 1 and Iba1 double stainings, Student’s t -test; n = 6 mice / group for IL-4 ELISA, repeated measures two-way ANOVA).
Digital Holographic Microscope Holomonitortm M2, supplied by Phase Holographic Imaging PHI AB, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+flag+m2+affinity+resin/holomonitortm+m2+digital+holographic+microscope/us09487561-331-6-11
Average 90 stars, based on 1 article reviews
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99
Thermo Fisher rpmi 1640 complete medium
(A) Representative double-immunofluorescence of CD16/32 (classical activation marker; red), CD206 or <t>arginase</t> <t>1</t> (ARG1, alternative activation markers; red) and Iba1 (green) in the contusion margin (see the red box in the brain atlas coronal brain section in Figure ) of WT and sEH KO mice at 1 day and 4 days following TBI. Arrowheads indicate the cells of colocalization. The inset images represent higher magnification of the boxed region in the corresponding images. The scale bar is 100 μm. The bar graph shows the degree of Iba1 and CD16/32, CD206 or arginase 1 colocalization in gray pixel intensity. Deletion of sEH resulted in a significant decrease of CD16/32-Iba1double-positive signal at 1 day and 4 days and an increase of CD206-Iba1 or arginase-1 double-positive signal at 4 days post-TBI. (B) Bar graphs of IL-4 protein level as assessed by ELISA in ipsilateral hemispheres of WT and sEH KO mice at 1 day and 4 days following sham surgery or TBI. Deletion of sEH caused a significant elevation of IL-4 level at 4 day. Values are mean ± S.E.M; ### , ††† P < 0.001 vs. sham group; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. WT group (n = 5 mice / group for CD16/32, CD 206 or arginase 1 and Iba1 double stainings, Student’s t -test; n = 6 mice / group for IL-4 ELISA, repeated measures two-way ANOVA).
Rpmi 1640 Complete Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
Thermo Fisher anti flag m2 agarose beads
(A) Representative double-immunofluorescence of CD16/32 (classical activation marker; red), CD206 or <t>arginase</t> <t>1</t> (ARG1, alternative activation markers; red) and Iba1 (green) in the contusion margin (see the red box in the brain atlas coronal brain section in Figure ) of WT and sEH KO mice at 1 day and 4 days following TBI. Arrowheads indicate the cells of colocalization. The inset images represent higher magnification of the boxed region in the corresponding images. The scale bar is 100 μm. The bar graph shows the degree of Iba1 and CD16/32, CD206 or arginase 1 colocalization in gray pixel intensity. Deletion of sEH resulted in a significant decrease of CD16/32-Iba1double-positive signal at 1 day and 4 days and an increase of CD206-Iba1 or arginase-1 double-positive signal at 4 days post-TBI. (B) Bar graphs of IL-4 protein level as assessed by ELISA in ipsilateral hemispheres of WT and sEH KO mice at 1 day and 4 days following sham surgery or TBI. Deletion of sEH caused a significant elevation of IL-4 level at 4 day. Values are mean ± S.E.M; ### , ††† P < 0.001 vs. sham group; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. WT group (n = 5 mice / group for CD16/32, CD 206 or arginase 1 and Iba1 double stainings, Student’s t -test; n = 6 mice / group for IL-4 ELISA, repeated measures two-way ANOVA).
Anti Flag M2 Agarose Beads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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Image Search Results


(A) Representative double-immunofluorescence of CD16/32 (classical activation marker; red), CD206 or arginase 1 (ARG1, alternative activation markers; red) and Iba1 (green) in the contusion margin (see the red box in the brain atlas coronal brain section in Figure ) of WT and sEH KO mice at 1 day and 4 days following TBI. Arrowheads indicate the cells of colocalization. The inset images represent higher magnification of the boxed region in the corresponding images. The scale bar is 100 μm. The bar graph shows the degree of Iba1 and CD16/32, CD206 or arginase 1 colocalization in gray pixel intensity. Deletion of sEH resulted in a significant decrease of CD16/32-Iba1double-positive signal at 1 day and 4 days and an increase of CD206-Iba1 or arginase-1 double-positive signal at 4 days post-TBI. (B) Bar graphs of IL-4 protein level as assessed by ELISA in ipsilateral hemispheres of WT and sEH KO mice at 1 day and 4 days following sham surgery or TBI. Deletion of sEH caused a significant elevation of IL-4 level at 4 day. Values are mean ± S.E.M; ### , ††† P < 0.001 vs. sham group; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. WT group (n = 5 mice / group for CD16/32, CD 206 or arginase 1 and Iba1 double stainings, Student’s t -test; n = 6 mice / group for IL-4 ELISA, repeated measures two-way ANOVA).

Journal: Oncotarget

Article Title: Deletion or inhibition of soluble epoxide hydrolase protects against brain damage and reduces microglia-mediated neuroinflammation in traumatic brain injury

doi: 10.18632/oncotarget.21139

Figure Lengend Snippet: (A) Representative double-immunofluorescence of CD16/32 (classical activation marker; red), CD206 or arginase 1 (ARG1, alternative activation markers; red) and Iba1 (green) in the contusion margin (see the red box in the brain atlas coronal brain section in Figure ) of WT and sEH KO mice at 1 day and 4 days following TBI. Arrowheads indicate the cells of colocalization. The inset images represent higher magnification of the boxed region in the corresponding images. The scale bar is 100 μm. The bar graph shows the degree of Iba1 and CD16/32, CD206 or arginase 1 colocalization in gray pixel intensity. Deletion of sEH resulted in a significant decrease of CD16/32-Iba1double-positive signal at 1 day and 4 days and an increase of CD206-Iba1 or arginase-1 double-positive signal at 4 days post-TBI. (B) Bar graphs of IL-4 protein level as assessed by ELISA in ipsilateral hemispheres of WT and sEH KO mice at 1 day and 4 days following sham surgery or TBI. Deletion of sEH caused a significant elevation of IL-4 level at 4 day. Values are mean ± S.E.M; ### , ††† P < 0.001 vs. sham group; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. WT group (n = 5 mice / group for CD16/32, CD 206 or arginase 1 and Iba1 double stainings, Student’s t -test; n = 6 mice / group for IL-4 ELISA, repeated measures two-way ANOVA).

Article Snippet: To assess proinflammatory (M1) and anti-inflammatory (M2) microglia/macrophages, sections were incubated overnight at 4°C with rabbit anti-Iba1 (1:1,000; Wako), together with rat anti-CD16/32 (a classical M1 activation marker; 1:100; BD Biosciences), mouse anti-CD206 (an alternative M2 activation marker; 1:100; Bio-Rad Laboratories, Hercules, CA, USA) or rabbit anti-arginase 1 (an alternative M2 activation marker; 1:100; BD Biosciences).

Techniques: Immunofluorescence, Activation Assay, Marker, Enzyme-linked Immunosorbent Assay