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Image Search Results
Journal: Oncotarget
Article Title: Deletion or inhibition of soluble epoxide hydrolase protects against brain damage and reduces microglia-mediated neuroinflammation in traumatic brain injury
doi: 10.18632/oncotarget.21139
Figure Lengend Snippet: (A) Representative double-immunofluorescence of CD16/32 (classical activation marker; red), CD206 or arginase 1 (ARG1, alternative activation markers; red) and Iba1 (green) in the contusion margin (see the red box in the brain atlas coronal brain section in Figure ) of WT and sEH KO mice at 1 day and 4 days following TBI. Arrowheads indicate the cells of colocalization. The inset images represent higher magnification of the boxed region in the corresponding images. The scale bar is 100 μm. The bar graph shows the degree of Iba1 and CD16/32, CD206 or arginase 1 colocalization in gray pixel intensity. Deletion of sEH resulted in a significant decrease of CD16/32-Iba1double-positive signal at 1 day and 4 days and an increase of CD206-Iba1 or arginase-1 double-positive signal at 4 days post-TBI. (B) Bar graphs of IL-4 protein level as assessed by ELISA in ipsilateral hemispheres of WT and sEH KO mice at 1 day and 4 days following sham surgery or TBI. Deletion of sEH caused a significant elevation of IL-4 level at 4 day. Values are mean ± S.E.M; ### , ††† P < 0.001 vs. sham group; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. WT group (n = 5 mice / group for CD16/32, CD 206 or arginase 1 and Iba1 double stainings, Student’s t -test; n = 6 mice / group for IL-4 ELISA, repeated measures two-way ANOVA).
Article Snippet: To assess proinflammatory (M1) and anti-inflammatory (M2) microglia/macrophages, sections were incubated overnight at 4°C with rabbit anti-Iba1 (1:1,000; Wako), together with rat anti-CD16/32 (a classical M1 activation marker; 1:100; BD Biosciences), mouse anti-CD206 (an alternative M2 activation marker; 1:100; Bio-Rad Laboratories, Hercules, CA, USA) or
Techniques: Immunofluorescence, Activation Assay, Marker, Enzyme-linked Immunosorbent Assay